The fully automated microbial detection system detects Escherichia coli and total coliform bacteria by using a widely accepted microbial enzyme-labeled indicator characteristic detection method. This technology has passed on-site testing and parallel comparative research with standard microbial analysis methods., widely certified. The fully automated microbial detection system uses free patented (based on polymer optical sensors) results and is achieved through "polymer optical partitions" built in each detection sleeve. Each detection sleeve contains a certain amount of specific culture medium to provide nutrients to target bacteria in the water sample. When target bacteria such as Escherichia coli begin to reproduce, a special enzyme is produced that reacts with a proprietary chemical medium in the sleeve, causing the medium to release fluorescent molecules. These fluorescent molecules act as indicators to quickly move from the water sample to the polymer optical sensor in the sleeve. There is an ultraviolet optical detection unit in the "Fully Automated Microbial Detection System" to achieve automated detection.