This project belongs to the technical field of plant quarantine. Gypsy moth is a serious omnivorous forest pest. It is widely distributed in northern China. In recent years, it has spread to Jiangsu, Zhejiang, Jiangxi and other places, just one step away from Shanghai, seriously threatening Shanghai's forestry and ecological security; At the same time, in order to restrict shipping trade in Shanghai Port, the North American Plant Protection Organization issued RSPM Standard No. 33 in 2009, which identified Shanghai Port as a high-risk port for the spread of Asian gypsy moths and required additional inspections of ships on North American routes passing through Shanghai Port. Gypsy moth ship quarantine will be restricted from entering the port or expelled from the country. Since then, Mexico, Chile, New Zealand and other countries have successively introduced similar restriction policies. As the world's largest port, Shanghai Port has an average annual cargo throughput of more than 700 million tons, of which ships on the North American route account for about 50%. If the restriction policy cannot be effectively responded to, it is expected to cause huge economic losses of US$50 billion and seriously affect Shanghai Port's status and reputation as an international shipping center. In order to fill the gap in gypsy moth ship quarantine technology as soon as possible and minimize the impact on Shanghai Port trade, this project starts with the research and development of testing technology, develops a three-dimensional monitoring and control system, establishes a highly targeted and efficient killing treatment technology, and at the same time Conduct a comprehensive assessment of the risk of gypsy moth being introduced into Shanghai, identify key control points, comprehensively establish an early warning mechanism and prevention and control system to prevent gypsy moth from being introduced into Shanghai, and solve the technical issues of ldquo; where it comes from, whether it exists, how to deal with it, and how to prevent it. Main innovation contents: 1. Creation of precise and rapid detection technology for gypsy moth. For the first time in the world, the complete mitochondrial sequence of gypsy moth was completed. On this basis, methods for detecting different geographical types of gypsy moth were established, and a geographical traceability method based on AFLP molecular markers was created to achieve rapid identification in 6 hours and accurate positioning in 10 hours;2. Establishment of efficient gypsy moth monitoring technology. For the first time in the world, the interaction mechanism between gypsy moth olfactory proteins and external information compounds was studied, clarifying the differences in olfactory between male and female insects, and revealing the molecular mechanism for gypsy moth to recognize external information compounds. Based on this, the high-affinity compound mdash;CA-074 methyl ester was discovered., and a new type of trap has been developed to comprehensively deploy and control high-risk ports;3. Research and development of gypsy moth quarantine treatment technology. For the first time, a fumigation treatment technical system for two fumigants, methyl bromide and sulfuryl fluoride, was established to kill different insect stages of gypsy moth. A track-type veneer reptile robot and a high-efficiency fumigant vaporization dosing device were developed, which solved the problem of difficulty in removing ship egg masses. Technical problems;4. Risk analysis and creation of management and control technology for the introduction of gypsy moth into Shanghai. For the first time, a prediction model for the distribution and hazard of gypsy moth in China and a risk assessment model introduced to Shanghai from general trade patterns were constructed, and key points for the introduction of gypsy moth into Shanghai were screened out. The project published 25 papers (6 SCI sources and 1 EI source), 2 treatises, formulated 3 industry standards, and obtained 6 nationally authorized invention patents and 4 utility model patents. Promotion and application: This project provides scientific basis for the establishment of a gypsy moth early warning mechanism and prevention and control system in Shanghai, and provides technical support for ship quarantine. In the ten years since the establishment of the prevention and control system, no gypsy moth has occurred in Shanghai, maintaining agricultural and forestry production and ecological security. 1. The relevant plans and technical regulations of the Shanghai government adopt the research results of this project;2. The formed monitoring, testing and processing patents and industry standards have been applied in the quarantine of gypsy moth ships. Including 58 ports in Shanghai and the country, 100% cover high-risk ports of gypsy moth. Provide technical support to resolve trade disputes arising from the carrying of gypsy moth;3. It has been applied in the monitoring and control of garden pests in Shanghai, ensuring the cleanliness of the city and forestry ecological security;4. It has taught more than 400 ship inspection personnel, providing technical consultation for the inspection of approximately 3000 ships every year to improve the technical level of employees.