This project belongs to the discipline of livestock parasitology. Schistosoma japonica is endemic to humans and animals and is seriously harmful. After the founding of New China, my country has achieved remarkable results in the prevention and control of schistosomiasis. However, due to difficulties in controlling the source of infection, the difficulty in eliminating intermediate host snails, and the influence of other natural and social factors, the epidemic situation did not decline significantly at the beginning of this century, and some areas even rebounded. Livestock is the main source of infection and the key to prevention and control of schistosomiasis in my country. This research is guided by the national prevention and control needs, and is based on& ldquo; human and animal prevention, and gate moving forward& rdquo; In principle, we collaborated to tackle key technical problems in controlling schistosomiasis epidemics in livestock, and achieved the following results: 1. It reveals the epidemic characteristics of schistosomiasis in livestock in my country since the beginning of this century, and clarifies the main reasons why the epidemic is difficult to reduce. On this basis, it proposes comprehensive prevention and control strategies and technical measures to cut off the transmission chain of schistosomiasis in livestock, control the source of infection in livestock, and combine agricultural production to suppress snail breeding in susceptible areas, as well as applicable prevention and control models for different types of epidemic areas and new models for investigation and treatment in lake and marsh epidemic areas; After demonstration and application in pilot areas, the infection rate of bovine schistosomiasis dropped from 0.6% to 20.1% in 2003 to 0% in 2016. The results were remarkable, breaking through the prevention and control dilemma of difficulty in declining the epidemic. 2. Six schistosomiasis diagnostic antigen molecules such as SjPGM were screened and obtained, and a multi-epitope recombinant diagnostic antigen was developed. The sensitivity and specificity of the established ELISA diagnostic technology for the diagnosis of sick cattle and sick sheep were 95.61%~97.8%, and the specificity was 97.8%~100%. The cross-reaction rate with other cattle and sheep parasitic helminths decreased by 66.68%~85.72% compared with the SEA-ELISA method. The sensitivity of the created colloidal gold rapid diagnostic test strip technology to bovine and sheep schistosomiasis is 100%, and the specificity is 93.24% and 88.64% respectively. Improve and improve the hatching method of fecal miracidia of livestock schistosomiasis, and the coverage rate of this method in epidemic areas reaches more than 95%. It breaks through the technical bottleneck of poor specificity of the original serological detection technology and low sensitivity of pathogen detection technology. A new preparation of praziquantel for the treatment of schistosomiasis with a small dose and killing effect on juvenile worms has been developed. Based on research on the functional genomics of schistosomiasis and the interaction mechanism between schistosomiasis and host, important worm molecules such as SjVAMP2 related to the growth and development of schistosomiasis were identified, and the molecular basis of differences in schistosomiasis infection between suitable host cattle and non-suitable host buffaloes was analyzed., laying the foundation for screening new drug targets and vaccine candidate molecules for schistosomiasis. 3. Propose the overall layout of schistosomiasis prevention and control in livestock in my country and form a comprehensive prevention and control system. The only epidemiological database of livestock schistosomiasis at home and abroad has been created. A series of comprehensive prevention and control technical specifications, guidelines and technical standards have been formulated. After the research results were fully promoted and applied in 1918 townships (towns) in 164 counties (cities, districts) in 7 provinces across the country, the infection rate of bovine schistosomiasis in 7 endemic provinces including Hunan, Hubei, Jiangxi, Anhui, Sichuan, Yunnan, and Jiangsu dropped from 0.13%~5.10% in 2003 to 0%~0.03% in 2016, a decrease of 98.49%~100% respectively, all meeting the transmission control standards and effectively controlling the epidemic of schistosomiasis in livestock. Contribute to the realization of the overall goals of the National Medium-and Long-Term Plan for Schistosoma Prevention and Control (2004-2015). The area of snails in the project implementation area decreased by 1.9644 million acres. At the same time, it reduced the losses caused by livestock infection with schistosomiasis, increased the income of agricultural schistosomiasis control projects, and increased the income of farmers in epidemic areas by 32.849 billion yuan. This project published 89 papers, including 31 papers included in SCI. Published 5 monographs. It has been authorized to have 7 national invention patents, 4 utility model patents, 3 software copyrights, and formulated 3 national and local standards. 25,000 people received technical training.