The R & D team extracted core antigen protein genes causing more than 70 diseases and more than 20 pests such as stem rot, downy mildew, mosaic, jujube madness, leaf blight, black rot, powdery mildew, red powder, gray mold, vine blight, charcoal rot, rust, blight, etc., and realized expression in Escherichia coli and Pichia pastoris, and developed more than 10 kinds of plant immune vaccine monomers (hypersensitivity proteins). On this basis, the team made multivalent combinations of more than 10 plant immune vaccine monomers to make the plant vaccine have anti-multiple pests and diseases. This project is suitable for the field of resistance to pests and diseases in crops and cash crops.
technical background
In the world, only two original invention patented technologies have been successfully applied in plant protection projects in the field of Messenger, a biopesticide that can induce resistance reactions, promote growth and development, is safe to humans and animals, and is harmless to the environment (one is the "Messenger" owned by EDEN Company in the United States, and the other is the "HhrpEcc Protein" owned by Israel's "Galile-Green" Laboratory). So far, plant vaccines (hypersensitivity proteins) have been recognized by the government both abroad and at home. However, the research and development of plant vaccines is only the tip of the iceberg, far from reaching the level of large-scale development.
technical principle
The R & D team extracted more than 70 diseases and more than 20 kinds of pests that cause stem rot, downy mold, mosaic, jujube blight, leaf blight, black rot, white powder, red powder, gray mold, vine blight, charcoal rot, rust, and blight, and developed more than 10 kinds of plant immune vaccine monomers (hypersensitivity proteins). These vaccines have good prevention and control effect on various plant diseases caused by viruses, bacteria, fungi and other pathogens. The control effect of pests and diseases is 40% to 80%, and can increase crop production by 10 to 75%. In actual use, the amount of pesticides and fungicides can be reduced by up to 50%. According to existing reports, our work is currently the most complete variety and broadest functional coverage of plant vaccines and immunostimulants in the world. On this basis, the team has made multivalent combinations of more than 10 plant immune vaccine monomers to make the plant vaccine have anti-various pests and diseases. For example, the Harpin ECB multivalent vaccine developed by us has been used for scab and leaf spot diseases., black powder, powdery mildew, and white blight, etc. all have significant joint prevention and control effects. According to reports, our work is unique and innovative, especially the industrialization idea of gene expression and ratio of multivalent (multiple) vaccines, which is the first in the world. In addition, we have also developed a series of plant immune stimulants using plant immune protein as one of the main raw materials, coupled with gamma-polyglutamic acid, fulvic acid, trace elements and other ingredients, and expanded the scale of market use. This part of the content also has high innovation and large market prospects.
application impact
Effect 1: After spraying the plant immune protein Harpin ECB, the corn plants were strong and tall, and the incidence of corn borers and myslists was reduced by 40-55%.
Effect 2: After spraying the plant immune protein Harpin ECB, the rice texture is smooth and plump, and the incidence of rice blast is reduced by more than 60%. The above are some examples of the test results.
technical advantages
(1) Compared with existing plant vaccines developed by the team, the new generation of plant vaccines represented by Harpin ECB

1. The structure is more optimized, the ability to recognize loci is stronger, and the titer is higher;
2. It mainly focuses on multivalent vaccines, which have significant joint control effects on multiple pests and diseases;
3. Using plant immune protein as one of the main raw materials, and adding gamma-polyglutamic acid, fulvic acid, trace elements and other ingredients, this part of the content is also the first;
4. The R & D team has established the most complete database of plant disease core antigens at home and abroad;
5. This research and development team has significant advantages in protein structure research. Since 2002, major national science and technology projects have funded this research and development team four consecutive times to carry out structural transformation of recombinant proteins, optimization of expression systems and optimization of fermentation conditions, with a total funding of more than 69 million yuan. A large number of recombinant protein drugs and enzyme preparations including TRAIL, Pediocin, GDI2, etc. have been launched, generating economic benefits of 2 billion yuan. In recent years, we have continuously carried out the research and development and industrialization of plant vaccines, and have successively launched a new generation of plant vaccines, multivalent vaccines and plant immunostimulants series represented by Harpin ECB, and are currently in the pilot stage of industrialization;
6. This series of products does not directly affect targets (such as pathogenic bacteria, viruses and insects), but stimulates the high expression of disease-related genes and proteins, making the plants themselves resistant, enhancing the synthesis of plant phytochemicals and lignin, and activating plant defense. Enzyme system improves plant immunity and prevents the invasion of bacteria. Compared with traditional pesticides, it has no residues and is harmless to humans and animals. It is a very green and environmentally friendly new generation of plant vaccines and immunostimulants.
   (2) The new generation of plant vaccines developed by the team, represented by Harpin ECB, is compared with the HarpinEcc and HarpinEa, which have just been launched.
7. The structure is more optimized, the ability to recognize loci is stronger, and the titer is higher;
8. Harpin ECB is a multivalent vaccine that has significant joint prevention and control effects on scab, leaf spot disease, black powder, powdery mildew, and white silk blight. This is an advantage that other products do not have. This part of the content is also the first in the world;
9. Using plant immune protein as one of the main raw materials, coupled with gamma-polyglutamic acid, fulvic acid, trace elements and other ingredients, we have developed a series of plant immune stimulants (non-pesticides) to expand the scale of market use. This part of the content is also the first.