The mortality rate of livestock and poultry in large-scale breeding is 8-15%. Dead livestock and poultry and slaughterhouse leftovers are scarce carbon and nitrogen raw materials in the manufacture of bio-organic fertilizers; my country produces 2 billion tons of straw every year, which is the best organic carrier for carbophilic Trichoderma fungi. Since ordinary organic fertilizers have poor effects in the current season and are not accepted by farmers, the development of all-purpose bio-organic fertilizers with comparable effects to chemical fertilizers in the current season is a major scientific and technological requirement for my country to implement the national policy of organic substitution for chemical fertilizers. Bacillus has become the main functional bacteria in conventional bio-organic fertilizers because it is easy to expand and produce spores. Trichoderma has large biomass, strong root surface colonization ability, and many types and content of secondary metabolites, which has more significant growth promotion and biocontrol effects. However, Trichoderma cannot effectively produce spores in liquid propagation. However, the traditional solid fermentation process has expensive raw materials and high material sterilization costs, making it difficult to expand scale, becoming a technical bottleneck in the Trichoderma bio-organic fertilizer industry. This achievement has invented a technical process for large-scale production of Trichoderma spores using straw and waste animal protein acidolysis amino acids as raw materials without sterilization of materials in an open space, breaking through the technical bottleneck in the manufacture of Trichoderma all-purpose bioorganic fertilizers.