This project belongs to the field of computational biology. This project uses a combination of experimental and computational analysis, through epigenetics, functional genetics and integrated network analysis, to deeply and extensively explore the mechanisms of development and aging, and to discuss premature and rapid aging caused by environmental and genetic factors. A series of major scientific issues such as related diseases are discussed. In the past ten years, the project has carried out persistent exploration in this important field and achieved a series of groundbreaking results, the specific contents are as follows: 1. The development of algorithms in development and aging research has established an improved accurate nucleosomes based on sequencing data. Positioning algorithm iNPS. Nucleosomes localization information obtained based on iNPS can help analyze changes in nucleosomes distribution signals during the occurrence, development, invasion and metastasis of malignant tumors. A semi-automated in situ hybridization image analysis process was developed. Using this process, spatial expression patterns of genes in the cerebral cortex were derived at single-cell resolution to provide insight into key events that occur during cerebral cortex development. 2. New progress has been made in the study of aging phenotypes. Genetic expression profile analysis of middle-aged mice with different dietary structures reveals aging regulatory factors. From the perspective of systems biology, it reveals the molecular mechanism by which diet regulates aging and provides new concepts for aging intervention strategies. By analyzing three-dimensional facial images, a physiological age prediction method based on three-dimensional facial images is constructed, and further quantitative image phenotypes, feature or marker selection, and phenotype-molecular feature correlation analysis are analyzed, laying a foundation for establishing a human development and aging database. foundation. 3. Intervention on development, aging and aging-related diseases. In terms of development, advanced technologies such as laser microdissection and single cell sequencing were used to draw a fine three-dimensional molecular map of the embryo in the middle stage of mouse early development. In terms of aging-related diseases, it has been found that the highly mutated gene UTX-1 in malignant tumors regulates aging through the insulin analogue signaling pathway. It is the first time to report the regulatory effect of histone modification genes that function through somatic cells on this important biological process of aging., deepening the understanding of epigenetic functions and providing potential targets for the development of new anti-aging drugs. An unbiased adaptive clustering method was developed to comprehensively analyze the panoramic view of the genome, epigenome and transcriptome of ovarian cancer, thereby revealing potential modularity characteristics of poor prognosis of ovarian cancer. The project published more than 30 papers, and 8 representative papers selected from them were published in journals such as Cell Metabolism, Developmental Cell, Cell Research, etc., and were cited 299 times in total, and he cited 220 times. The first person to complete the project, Han Jingdong, was awarded the 100-Person Plan of the Chinese Academy of Sciences, the National Ten Million Talents Project, the Young and Middle-aged People with Outstanding Contributions, the MaxNetAging Fellow, and the Faculty 1000 in Developmental Biology. The first finisher organized two international systems biology conferences in 2008 and 2011. In 2013, the first Cold Spring Port Asian ldquo;Molecular Basis of Aging and Disease international conference was organized and held in Suzhou. The International Aging Research Summit will be held in 2017 and 2018 and participated in many international and domestic academic conferences. He has published academic reports nearly 100 times at international academic conferences and world-renowned universities or research institutions (such as UC, MPI, etc.), and has gone to the United States, Japan, Singapore and other places to participate in international academic conferences and conduct cooperation and exchanges with internationally renowned experts.