This project belongs to the field of food science and agricultural and sideline product processing. The content covers the deep processing of agricultural and sideline products and the development and application of related high value-added products.
my country's annual output of tea reaches more than 1.6 million tons, of which coarse and old tea accounts for about 25%, which is often abandoned or abandoned for processing; a large number of branches, leaves, camellia, and tea seeds produced by pruning tea trees are also abandoned every year. Therefore, how to develop coarse old tea, camellia and tea seeds has become a top priority for the development of my country's tea industry. This project uses coarse tea leaves, camellia and tea seeds as raw materials to extract tea-derived polysaccharides from them and study their properties and applications. Aiming at tea-derived polysaccharides, we studied the integrated technology of extraction, separation, purification and drying to protect their activity. Focusing on the structure/function of tea-derived polysaccharides, we carried out research on qualitative and quantitative control technology of tea-derived polysaccharides from three aspects, including: establishment of structural qualitative analysis technology, fingerprint qualitative analysis technology and quantitative technical system for tea-derived polysaccharides; At the same time, its biological activity was evaluated to prepare tea polysaccharide products with good biological activities (such as hypoglycemic activity, skin care activity, etc.), and tea polysaccharide was coordinated with metal elements such as calcium, iron, and selenium to obtain tea polysaccharide complex. We also extracted natural selenium-rich tea polysaccharides from selenium-rich green tea in nature and applied it in the development of baked foods, cosmetics and other products. The key innovative technologies of this project are reflected in:
(1) On the basis of obtaining highly bioactive tea source (tea, camellia) polysaccharides, using activity-protected integrated biological extraction, separation, purification and drying technology, tea source polysaccharides with high activity and high yield can be produced on a large scale. Tea polyphenols can also be comprehensively extracted;
(2) The production process of simultaneously extracting tea seed polysaccharides and tea saponin by alcohol and water extraction was studied;
(3) A multi-fingerprint qualitative detection method for tea polysaccharide (TPS) was established for the first time;
(4) The quantitative determination method of tea polysaccharides was improved using the monosaccharide composition of tea polysaccharides as a mixed standard;
(5) A new resonance light scattering method for the determination of tea polysaccharide content based on the TPS-CPC-NaOH system was established for the first time;
(6) Extract natural selenium-containing tea polysaccharides, and apply this selenium-rich matcha to the development of baked goods, cosmetics and other products;
(7) Combining the moisturizing effect of tea polysaccharides with the antioxidant effect of tea polyphenols, new products such as selenium-rich tea polysaccharide cosmetics (selenium-rich tea polysaccharide matcha mask, tea polysaccharide skin care lotion) have been developed for the first time.
This project is the result of the National 863 Plan Project, the Shanghai City Venus Project, the Shanghai City Venus (Tracking) Project, the Shanghai City International Cooperation Project, and the Shanghai City Education Commission Key Scientific Research and Innovation Projects hosted by this research group. The project has published more than 30 SCI papers in international journals, and 3 China invention patents have been authorized for this technology. "Pilot preparation of high-purity camellia polysaccharides and development of hypoglycemic functional foods" won the "Venus Star" Talent Award of Shanghai City (2007),"Comprehensive extraction technology of tea polyphenols and tea polysaccharides" won the second prize for outstanding exhibits in the China university exhibition area at the 2010 China International Expo (2010),"Comprehensive Development and Utilization of Tea Resources" won the second prize for outstanding exhibitors at the 2010 Eighth Straits Project Achievements Fair (2010),"Research on the structure-activity Relationship between Tea Polysaccharide Structure and Glucosidase Inhibitory Activity" won the "Venus Star" Tracking Talent Program Award of Shanghai City (2011), and "Tea Polysaccharide Chemistry and Functionality" won the first prize of the 3rd China Tea Society Science and Technology Award (2013). The project provides relevant technical and theoretical support for the efficient and comprehensive utilization of coarse and old tea resources, the production and application of tea-derived polysaccharides, and the development of the tea industry. It has been demonstrated and developed in Shanghai Yuanzumeng Fruit Co., Ltd. and Sichuan Province Kroni Tea Industry Biotechnology Co., Ltd. In the past three years, the products have achieved a cumulative new output value of more than 1 billion yuan, an additional profit of more than 300 million yuan, and an additional tax revenue of nearly 100 million yuan, which has produced good economic and social benefits.