This project belongs to the field of food science and technology. Aminooligosaccharides are the only cationic oligosaccharides in nature widely used in medicine, food, agriculture, materials and other fields. They have important physiological activities such as anti-cancer and immune induction. Commercial aminooligosaccharides are obtained from marine chitin resource-crab shells. Acid and alkali treatment to prepare glycans and then enzymatically hydrolyzed. The bottlenecks faced by the industry are: ① Traditional processes consume a large amount of acid and alkali and discharge a large amount of high-salt wastewater, which is difficult to treat and extremely serious environmental pollution;② Oligosaccharide products have a wide molecular weight distribution range, complex components, unstable quality, and unclear efficacy;③ Oligosaccharide stability is poor and formulated product development is difficult. Breaking through the above bottlenecks and establishing key technologies to improve the quality and efficiency of aminooligosaccharides are key issues in promoting the development of the marine ammonia sugar industry.
In response to the above problems, based on molecular scale research on raw materials, products and impurities, material physical properties and flow field control, the above problems have been solved through techniques such as enzyme catalysis, membrane separation, chromatographic medium construction and group modification. The main invention points are as follows:
① Invented a high-density steam flash explosion coupled enzyme membrane high-quality closed-loop production technology. Based on the analysis of the molecular scale of the shell structure, 10MP high-density steam is used to instantaneously relieve the pressure for 0.0875 seconds and accurately flash pre-treat the raw materials, completely opening the dense crystal structure of the shell and chitin, allowing the complex enzyme to more completely extract the protein and obtain a uniform molecular weight distribution. Aminoglycans, greatly reducing the amount of acid and alkali. The relationship between flow field characteristics and mass transfer efficiency, flow field distribution and other related characteristics in the flow channel during membrane recycling wastewater is clarified. Based on computational fluid dynamics simulation, real-time control of the macro membrane system and flow channel micro-flow field characteristics is realized to completely solve the problem of membrane pollution., realizing closed-loop high-value comprehensive utilization of amino oligosaccharide production process.
② Invented the preparation technology of amino oligosaccharide monomers and the preparation technology of neutral amino oligosaccharides. Strains that metabolize and produce amino oligosaccharides with high enzyme activity and specific catalytic hydrolysis were screened and constructed, and the hydrolysis technology synergistic with non-specific enzymes was optimized; the spatial conformation characteristics and molecular structure characteristics of amino oligosaccharides with different degrees of polymerization were studied. Through molecular modification and material property control technology, an amino oligosaccharide membrane separation technology based on molecular modification and property control was created; Special chromatographic media and special ion exchange resin have been built, and new intelligent online feedback control continuous chromatography equipment for multi-scale process optimization has been designed. It has taken the lead in realizing kilogram preparation of amino oligosaccharide monomers based on membrane and chromatography and industrialization of neutral amino oligosaccharides. Scale production.
③ Break through multiple technical problems in the stability and application of amino oligosaccharides. Technologies such as continuous chromatography ion displacement, positioning molecule modification, and negative pressure deoxy group protection have been developed, which have broken through the stability of aminooligosaccharides and the stability of the combination of aminooligosaccharides and chemical fungicides.
Based on the above inventions, new products and new processes with innovative and independent intellectual property rights have been obtained, filling many technical gaps. Production wastewater discharge is less than 5%, liquid alkali recovery rate is ≥95%, protein recovery rate is ≥95%, alkali consumption is reduced by 75%, and hydrochloric acid consumption is reduced by 30-35%; DP6 -8 Aminooligosaccharides are industrialized, the product contains DP6 -8 sugar content is ≥85%, and the monosaccharide content is ≤3%; the kilogram level of aminooligosaccharide monomers is prepared, with the monomer purity is ≥98%; the neutral aminooligosaccharide preparation yield is ≥92%, and the product pH range is 6-8; The developed 5% amino oligosaccharide plant immunity inducer increases crop yield by 5-15% on average.
Through 8 vertical projects, 25 invention patents were applied for (12 authorizations); chitooligosaccharide was approved for new food raw materials and obtained my country's first chitooligosaccharide production license; 4 aminooligosaccharide pesticide registration certificates were obtained; 1 national standard was established.
Achievements Since 2010, industrialization has been implemented in 5 enterprises. Due to the new products produced by this technology, as well as the value-added brought by cleaner production and comprehensive utilization, and improved technical indicators, the new output value has reached 943 million yuan in the past three years, and the new profits and taxes have increased. 225 million yuan, with significant economic and environmental benefits. This technology can be widely extended to the production of more products in the field of light chemical industry.