field of science and Technology
The technology of this project involves many fields such as microbial technology, fermentation engineering, biochemistry technology, biostatistical technology and experimental zoology. It is a comprehensive application of modern biotechnology, engineering and nutrition.

main content
This project develops an innovative probiotic feed additive with antioxidant function based on oxidative stress and intestinal barrier dysfunction in piglets during weaning. It improves the antioxidant capacity of piglets to alleviate oxidative damage occurring during weaning. Protect the intestinal barrier function and improve the immunity of piglets to change the current method that mainly relies on antibiotic drugs.

Key innovation points

1. Screening, optimized combination and culture technology of high-expression antioxidant bacteria are isolated from animal intestines, molecular biology technology is used for screening and optimization, and the stability, tolerance and activity of the bacteria are improved through targeted optimization of the bacteria and coating process., and production efficiency are improved.
2. Establishment and optimization of a step-by-step combination fermentation process for multi-strain microorganisms. Multiple strains survive and reproduce together under the same culture conditions; hierarchical fermentation under the overall fermentation process. The purpose of the primary fermentation process is the proliferation and metabolic activity of microorganisms. Generation of enzymes; the main products of secondary fermentation are a variety of antioxidants; and finally, the process of re-proliferation of microorganisms.
3. Protection and repair technology of intestinal barrier function in piglets. Use the antioxidant function of the product and the microflora regulation function of probiotics to remove excessive free radicals produced during weaning of piglets and protect the intestinal barrier function.

Patents
A special fermented feed for piglets and its preparation method (201110316531.8)

Thesis

1. Feed industry "Research Progress on Intestinal Barrier Function of Weaned Piglets"
2. Scientific experiments and research "Effect of compound antioxidants on the main intestinal flora of early-weaned piglets"

Promote and apply

1. At present, a new production workshop of 2500 square meters has been added in Shanghai Songjiang Export Processing Zone. In full accordance with the designed and verified production process, workshops such as fermentation, centrifugation, mixing, drying, cultivation, screening and packaging have been established to ensure the smooth progress of production. An 800-square-meter technology research and development center has been established in Minhang Xinzhuang Industrial Zone to ensure the smooth implementation of project research.
   On the existing basis, the daily trial production volume at the beginning of the project is about 300 kilograms; now the pilot production line has been completed, and the daily output can reach more than 2500 kilograms.
2. The company has established demonstration bases in Songjiang, Chongming, Minhang and other places, and has maintained long-term good cooperative relations with Guangming Chuandong Farm, May 4th Farm and cooperatives in Minhang District.