Key technologies and applications for biological identification and rapid detection of multiple pesticide residues in food
Innovative enzyme biosensor for rapid and broad-spectrum detection of pesticide residues, high automation, stability and low price, filling in pyrethroid
Type
Tags
Applicable industry
Applications
Key innovations
This technology innovatively develops biological enzymes and coupling systems that can simultaneously detect organophosphorus, carbamate and pyrethroid pesticides, filling the gap in rapid detection of pyrethroids.
Potential economic benefits
This pesticide rapid detection technology significantly improves detection efficiency and accuracy, expands detection range, reduces the cost of sensitive elements (0.5-0.6 yuan/root), and fills the gap in rapid detection of pyrethroid pesticides. Promotion and application have achieved a cumulative new output value of 40.31 million yuan, new profits of 15.528 million yuan, and new taxes of 2.761 million yuan in three years.
Potential climate benefits
Accelerate the testing of agricultural residues, reduce food waste due to excessive standards or long testing cycles, and indirectly reduce the carbon footprint of agricultural production, processing and waste disposal.
Solution supplier
View more
University of Shanghai for Science and Technology
Shanghai is a key university that cultivates outstanding engineering and technical talents to serve national strategies and regional development.
Shanghai，China
Solution details
- Technical field
Food Science and Technology - Main content
Most of the existing rapid detection methods for pesticide residues are based on the principle of enzyme inhibition to detect organophosphorus and carbamate pesticides, and there are few rapid detection methods for pyrethroid pesticides. In addition, problems such as limited sources and high prices of existing rapid detection sensitive enzymes, poor detection stability using liquid enzymes, vulnerability to sample color interference, and low degree of automation in the detection process have also seriously restricted the popularization and application of rapid detection technology for pesticide residues. In response to these issues, the main research contents and key inventions of this project are as follows:
(1) Based on the specific inhibition of enzymes by pesticides and the principle of enzyme directed catalysis of pesticide hydrolysis, a biological enzyme that can simultaneously identify organophosphorus, carbamate and pyrethroid pesticide residues has been innovatively developed, and an enzyme coupling system that can effectively amplify and detect the thermal response related to pesticide detection has been constructed, and the corresponding detection system has been screened, thus solving the problem that current rapid detection methods can only detect organophosphorus and carbamate pesticides. Improve the versatility of rapid determination methods for pesticide residues;
(2) Based on the biochemical characteristics of biological enzymes, a new technology for the preparation of immobilized biological sensitive elements and coupled enzyme sensitive elements that integrates purification and immobilization and is suitable for rapid biosensing detection has been established. Combined with nanomaterials to assist characterization, it overcomes the defect that electrostatic adsorption immobilized enzymes are easy to fall off, and makes it into a disposable enzyme column that is easy to replace. It solves the problem that the background color of food sample solution seriously interferes with the detection results due to the use of liquid-sensitive enzymes in the current rapid detection method of pesticide residues;
(3) Innovatively designed a pesticide residue detection method system that uses the same type of inactivated enzyme as a reference to eliminate non-specific interference in the system. At the same time, it combines a micro liquid flow distributor that can eliminate uneven flow distribution and a micro reactor that automatically updates solid carrier technology., using the close cooperation of immobilized biological sensitive elements with different transducers, a series of biosensor-type pesticide residue rapid detectors integrating automatic sample injection, online reaction, testing and result determination have been built. The automation and generalization of pesticide residue testing have been realized. - Project characteristics
(1) The project was authorized for 9 invention patents, applied for 5 invention patents, and published 57 related papers in core journals at home and abroad;
(2) Innovatively developed biological enzymes that can simultaneously identify organophosphorus, carbamates and pyrethroid pesticide residues, expanding the source of sensitive enzymes for rapid detection of pesticides with high sensitivity, stability and low cost, filling the current gap in rapid detection. The method cannot detect pyrethroid pesticides;
(3) A new technology for the preparation of immobilized biological sensitive elements integrating purification and immobilization was studied. The cost of a single pesticide rapid detection sensitive element is only 0.5-0.6 yuan;
(4) Using the same inactivated enzyme as a reference to eliminate non-specific interference in the system, the entire flow path of the enzyme biosensor is temperature-controlled, and the operation is highly automated, and the parallel relative standard deviation is controlled within 7%;
(5) The newly constructed enzyme biosensor type pesticide residue rapid detector has a pretreatment time of 5-6 minutes and a detection time of 15 minutes. The detection limit for organophosphorus and pyrethroid pesticides reaches 0.5 mg/kg, and for carbamate pesticides reaches 1mg/kg. - Promote and apply
The project's technical achievements have been applied in Shanghai Agricultural Information Co., Ltd., and some technologies have been promoted at the pesticide testing point of Shanghai Testing Center, the testing center of Shanghai Pesticide Institute, Guangming Group Urban Vegetable Garden Distribution Center and Zhengyi Gardening Company. The series of immobilized sensitive elements, test supporting reagents and biosensors for rapid pesticide testing produced and used using this project technology have a cumulative new output value of 40.31 million yuan, a new profit of 15.528 million yuan, and a new tax revenue of 2.761 million yuan in the three years from 2011 to 2013.
Last updated
11:34:21, Nov 04, 2025
Information contributed by
See original page on
Report