This project belongs to the discipline field of plant protection. At present, 70% of biopesticides and biofertilizers in the world contain Trichoderma, which has become a core resource for reducing or replacing the use of chemical pesticides and fertilizers in agricultural production and building a green production system. Studies have shown that Trichoderma can not only replace or reduce the use of chemical pesticides and chemical fertilizers through its biological control function, but also have the function of repairing soil contaminated by agrochemical substances. However, for a long time, research on the screening and evaluation technology of Trichoderma biocontrol resources, the creation and large-scale production technology of high-spore-content inoculants, conidial protection technology, and the mechanism of biocontrol have been significantly lagging behind, which has greatly limited Trichoderma biocontrol. The development of technology. With the support of national 863 and other projects, this project has overcome the above problems through innovation and integration of biotechnology and engineering technology, and achieved the following innovative results:

1. Establish Trichoderma resource identification and antagonistic screening and evaluation techniques. The automatic retrieval system for the morphological and molecular identification of Trichoderma has filled the international gap. Among the 8751 Trichoderma strains isolated in ecological areas across the country, 37 were identified and 1 new species was discovered. For the first time, a rapid screening method and comprehensive evaluation model for biological control of Trichoderma based on the survival rate of the pathogen and the activity of cell wall degrading enzymes were established, and 905 antagonistic strains were obtained, 11 of which were industrially applied; a new method for predicting antagonistic secondary metabolites with molecular docking technology was established.
2. Explore and utilize new functions of Trichoderma genes. Through the construction of mutant strains, 13 genes were cloned, and new genes for inducing resistance elicitors and receptors were discovered; Trichoderma was clear to tolerate and degrade three organic phosphorus pesticides, three triazole fungicides, three herbicides and adsorption. New functions such as two heavy metals; for the first time, a bifunctional or bivalent engineering strain for Trichoderma was constructed for the first time.
3. New technologies to create and extend shelf life of inoculants with high spore content. Establish new technologies for enriching spores such as cyclone separation and water washing, with the spore content of the parent drug reaching 70 billion/g; through large-scale solid fermentation technology integrated with technologies such as solid koji making and sterilization, automatic cultivation control, efficiency is improved by 150-200%, and cost is reduced by 30%. For the first time, the functional group modification of the parent structure of Trichoderma was completed and a series of derivatives were obtained. A technology for efficient production of chlamydospores by liquid fermentation and post-feeding has been established. New technologies for conidia protection and microencapsulation allow the product to have a shelf life of more than 12 months. Developed 18 Trichoderma products and 21 combined use technologies.
4. Reveal the new mechanism of Trichoderma biocontrol and tolerance or degradation of agrochemical substances. For the first time, it was clear that Trichoderma induced systemic immunity in plants conforms to the MAMPs/DAMPs model, and it was found that Trichoderma's regulation of soil microecosystems is an important mechanism for preventing and controlling soil-borne diseases in corn and vegetables. It reveals the mechanism of Trichoderma resisting or degrading organic phosphorus pesticides and Jinggangmycin, adsorbing heavy metals, and relieving soil salinization stress.
   This project has obtained 26 national invention patents, 1 software copyright, 9 pesticide registration certificates, and 3 microbial fertilizer registration certificates. It has published 36 SCI papers and 153 domestic core journals, with a total of 1127 citations. Published 2 monographs in Chinese and 2 monographs in English (participating editors). Shanghai Dajing Bioengineering Co., Ltd. and Shandong Tainuo Pharmaceutical Co., Ltd. have used the above new technologies to improve the quality of biocontrol agent products, with an added output value of 82.8 million yuan and an added profit of 52.92 million yuan (direct economic benefits). In the past three years, Trichoderma biological control technology has been promoted on a large scale of 20.5 million acres across the country, with a control effect of 60%-96%, an increase in production of 9-20%, a reduction in soil salinization and heavy metal pollution by 30-40%, and chemical pesticides and chemical fertilizers were reduced by more than 30%, with an added output value of 9.32 billion yuan and an added profit of 4.688 billion yuan (indirect economic benefits). This project has led the development direction of using Trichoderma resources to biological control plant diseases in my country, and has achieved significant economic and ecological benefits.