The global burden of disease shows that fine particulate matter (PM2.5) in the atmosphere caused 2.94 million deaths in 2017. For every 10 micro;g/m3 increase in PM2.5, the risk ratio of cardiovascular metabolic disease (CMD) death was 1.12. However, its mechanism of action is not clear, and there are no effective prevention and control measures. This project focuses on the immune-inflammatory damage response and expounds the mechanism of PM2.5 causing CMD. At the same time, vitamin E and fish oil are used to intervene to provide new pharmacological targets and effective prevention and treatment measures for the treatment and prevention of PM2.5-related CMD. 1. Establish Asia's first ldquo; dynamic inhalation exposure laboratory for air pollution and a systematic method for toxicological research on PM2.5. Using this exposure system, the way animals are exposed to PM2.5 is consistent with the way humans actually inhale and expose PM2.5. Studies have found that short-term and long-term exposure to PM2.5 can lead to the formation of risk factors such as dyslipidemia, increased blood sugar, insulin resistance, increased arterial plaque, and myocardial damage. The results were published in Toxicol Lett, Int J Cardiol and others. Many domestic scientific research institutes have used this exposure system to conduct PM2.5 research, including the Yangtze River Delta Meteorological Environmental Warning and Forecasting Center and the Shanghai City Center for Disease Control and Prevention; many domestic enterprises and institutions such as Zhejiang Qiushi Artificial Environment Co., Ltd. have used PM2.5 toxicology research methods and technologies to build a meteorology-environmental animal exposure system. 2. Clarify the immune-inflammatory mechanism by which PM2.5 promotes the occurrence and development of CMD. This project established atherosclerosis models, type 2 diabetes models, hypertension models and normal animal models, and found that PM2.5 caused inflammatory reactions in the circulation system, myocardial tissue, and adipose tissue, manifested by the increase of pro-inflammatory factors IL-17 and ICAM-1 and the decrease of anti-inflammatory factors IL-10 and INF-gamma; at the same time, the release of inflammatory factors promoted the differentiation of macrophages into pro-inflammatory M1 cells, and CD4 +T cells differentiated towards pro-inflammatory Th17 and Th2 cells; and the activation of immune cells further led to the release of these inflammatory factors, which jointly promoted the occurrence and development of CMD. Therefore, cellular immunity or inflammatory factors can be used as early screening indicators or signals to monitor the impact of PM2.5 on CMD, and the results were published in Int J Cardiol and other journals. 3. For the first time in China, vitamin E and fish oil were used to intervene on cardiovascular harm caused by PM2.5. Studies have found that nutrient supplementation has a significant protective effect on the health hazards of the cardiovascular system caused by PM2.5, especially the simultaneous supplementation of an appropriate proportion of vitamin E and fish oil has a significant synergistic effect. The results show that nutrient supplementation can improve the body's immune function and antioxidant and anti-inflammatory capabilities to reduce the health hazards of PM2.5, which is of great significance for the protection of key groups, the establishment of comprehensive intervention strategies and the guidance of clinical nutrient intervention. The results were published in journals such as Plos One and Chemosphere. This project detects early monitoring signals of high-risk CMD (myocarditis, coronary heart disease, type 2 diabetes, etc.) caused by PM2.5 from the toxicological perspective. A total of 26 papers were published, with a total of 297 citations, and 5 doctoral students and 9 master's students were trained. The person in charge and members were invited to report at the ASIATOX2018 Asian Toxicology Conference, the 6th Asian Megacities Environment and Public Health Forum and other conferences; this project has also attracted the attention of ldquo; China Nutrition Society rdquo; and ldquo; China Environmental Sciences Society rdquo;. The established standard method for toxicological research on PM2.5 has been applied by many domestic scientific research institutes.