Turbot (commonly known as turbot) is a kind of marine fish loved by China people, and it is also the leading species of marine cultured fish in China. Edwardsiellosis is the primary disease endangering the healthy breeding of turbot. Currently, there is no effective treatment, with an incidence rate of as high as 50%, and a mortality rate of nearly 30%. Chemical drug treatment causes the emergence of multidrug resistant pathogens, threatening food and ecological security. Creating a vaccine for Edwardsiella and achieving disease immune prevention and control is of great significance to promoting the green development of turbot breeding industry. 1. The genus Edwardsiella was reclassified through comparative genomics and it was found that the pathogen of Edwardsiella turbot Edwardsiella was fish killing. Establish the most complete Edwardsia pathogen bank at home and abroad, including 103 typical strains with different characteristics; the genome sequencing of different representative strains was completed for the first time at home and abroad, and the genus was reclassified into five species through comparative genomic analysis. It was found that the pathogen causing Edwardsiella piscoides in my country was Edwardsiella; Develop a highly sensitive immune colloidal gold test strip that can directly collect ascites from diseased fish and complete the color reaction within 1 minute, achieving rapid on-site diagnosis of turbot Edwardsiella disease. 2. Clarify the molecular mechanism of the interaction between Edwardsiella fishkilling and the fish immune system, laying a scientific foundation for the design of live bacterial vaccines. It was found that the key virulence elements of Edwardsiella piscicida were type 3 secretion system (T3SS) and type 6 secretion system (T6SS), which could inject a variety of virulence effectors and interfere with cell functions. The infection mechanism of fish immune signal pathway controlled by important virulence factors of Edwardsiella piscicida was clarified. Caspy2 receptor molecule for fish to recognize pathogenic infection was identified and its anti-infection effect of inducing cell death was analyzed. He published 6 papers in Cell Host amp; Microbe, Nature Communications, mBio, PLoS Pathogens and other high-level journals, among which 1 was reviewed by CeLL sub-journal. 3. The invention of a new national veterinary drug turbot Edwardsiella live vaccine sets a milestone in the application of marine fish vaccine in China. EIBAV1 strain was found to be an excellent strain for developing attenuated live vaccine due to natural deletion of T3SS/T6SS gene cluster. Quality inspection system and operating procedures for marine fish vaccine were established to prove that the injection immunity protection rate of EIBAV1 strain exceeded 70%, and the immunity duration was 4 months. The national Class I new veterinary drug certificate was approved. Establish a field application protocol for a live vaccine against Edwardsiella maximus and promote it on a large scale in demonstration enterprises, reducing the incidence rate by 10-40% and reducing the amount of chemical drugs by 50-70%. This achievement has completed the entire chain of innovation and practice from pathogen identification, genome analysis, explanation of pathogenic mechanism to vaccine design and development for Edwardsiella disease, which seriously endangers turbot breeding in my country. It has published 60 SCI research papers, and he cited 740 times., a single article has a maximum of 79 other citations, and has been authorized 10 China patents, 1 national first-class new veterinary drug certificate, and 2 production document numbers. 2016-2018 During the year, a total of 42.4 million vaccines were promoted (2.8 million in 2016, 7.5 million in 2017, and 32.1 million in 2018), with more than 300 radiation breeding users. Among them, the promotion volume in 2018 has accounted for more than 30% of the total cultivation of turbot in the country. The promotion and application of vaccines has reduced the economic losses caused by Edwardsiella disease, reduced the amount of antibiotics in the turbot breeding industry, and made positive contributions to promoting green industrial development, ecological environment protection, and food safety.