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Mannanase and its application in petroleum exploitation
The new high-temperature resistant biological enzyme accurately breaks gel and increases the production of low-permeability oil fields.
Type
Enzyme preparation
Tags
Green mining of mineral resources
Energy
Thermophilic mannanase
Thermophilic bacteria
Gel breaking activity
High temperature resistant biological enzyme gel breaker
Water-based fracturing fluid gel
Solution maturity
Mass promotion / Mass production
Cooperation methods
Joint venture cooperation
Face-to-face consultation
Applicable industry
Scientific research and technology services
Applications
Oil exploitation
Key innovations
The innovation of this project lies in the development of a highly active thermophilic mannanase cloned from thermophilic bacteria. Its uniqueness lies in its high gel breaking activity at 80 ° C and low gel breaking activity at room temperature, effectively preventing the fracturing fluid gel from breaking prematurely.
Potential economic benefits
The high-temperature resistant biological enzyme can improve oil production efficiency and output in low-permeability oilfields, reduce import dependence and oil production costs, and has significant economic benefits and market potential.
Potential climate benefits
Improve oil production efficiency and reduce energy consumption and resource waste in single barrel of crude oil production. Biological enzymes replace traditional chemicals and reduce carbon emissions during the production, transportation and use of preparations.
Solution supplier
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East China University of Science and Technology
East China University of Science and Technology
East China University of Science and Technology: Focus on the intersection of multiple disciplines such as chemical industry and materials, cultivate innovative talents, and serve national strategies and social development.
Shanghai,China
Solution details

Project introduction: With the improvement of oil exploration and development in my country, the proportion of reserves in low-permeability oilfields is increasing. In the current situation of tight reserve reserves, better development of low-permeability oilfield reserves is of great practical significance to the sustainable and stable development of my country's petroleum industry. In order to increase the oil well production of low-permeability oil and gas reservoirs, high-pressure water-based fracturing fluid gel containing proppant can be injected into the oil well. Under high pressure, the oil-bearing rock formation will be fractured to create gaps. The fracturing fluid gel carries the proppant into the fractures to support it, and then the oil well is closed to crack the fracturing fluid gel under the action of the gel breaker. Then the cracked fracturing fluid is discharged back from the well. Due to the presence of proppant, cracks with good conductivity are formed in the oil layer. This will help increase oil well production.

Main features: This project isolated a thermophilic bacterium from soil and cloned it to obtain a highly active thermophilic mannanase. The half-life of the enzyme at 80℃ is 46 hours. Commodification of imports

Compared with the high-temperature resistant biological enzyme breaker Pyrolase®160, our mannanase has the outstanding advantages of good breaking activity at high temperatures and low breaking activity at room temperature (less than 5% of that in an 80℃ environment). This can effectively prevent the gel of the prepared water-based fracturing fluid from breaking prematurely before reaching the predetermined position, reducing the fracturing effect.

Technical level: The technology is at the leading level in China.

Application prospects: At present, the proportion of reserves in domestic low-permeability oilfields has reached more than 60%. Applying the high-temperature resistant biological gel breaker enzyme preparation we developed to the development of domestic high-temperature and low-permeability oilfields will have good increase production effects and economic benefits.


Last updated
11:47:35, Nov 05, 2025
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