The invention belongs to the technical field of biological analysis and detection, and particularly relates to a microfluidic chip for enriching microorganisms in the air and a preparation method thereof. The chip uses a molding method and uses optically transparent polydimethylsiloxane as a material. The chip contains sample enrichment channels and gas channels to collect bacterial, fungi, viruses and other biological particles in air samples. The enriched samples can be combined with PCR microfluidic chips and immune microfluidic chips for genetic analysis or immune analysis to achieve rapid detection of the content and types of microorganisms in the air.

The invention has the characteristics of being fast, efficient, portable, low-cost and easy automatic control. It can be integrated with other chips to complete automatic signal collection, remote transmission and signal analysis, and can be applied to on-site rapid detection of microorganisms in the air or other gases. Remote detection over a large range.