This project belongs to the field of medical support for diving operations. Marine strategy is one of the national development strategies. Diving technology is needed to complete marine resource development, military operations, submarine rescue and underwater construction. If the diver fails to implement decompression according to the prescribed plan due to harsh underwater working environment, operating errors, etc., decompression sickness will occur. In previous major projects in my country, decompression sickness occurred from time to time, which had adverse effects on national economic development. Therefore, there is an urgent need to carry out research on prevention and control measures for decompression sickness. Guided by the development of the national ocean strategy and supported by major national and military research plans, this project studies the pathogenesis, preventive measures, on-site first aid measures and key technical issues of decompression sickness, and has achieved a series of innovative results: 1. Innovatively, an experimental device with independent intellectual property rights for accurately controlling rapid pressure increase and decompression has been established, providing a reliable experimental platform for research on the pathogenesis of decompression sickness and its prevention and control measures; 2. Using a technical route combining theoretical calculation, simulation research and animal experimental verification, stable animal experimental models of decompression sickness caused by rapid compression and decompression were established for the first time. It was found that the damage caused by rapid compression and decompression reached its peak at 0.5 hours and 12 hours after decompression respectively. Severe decompression sickness mainly consists of acute lung injury and hemodynamic disorders, providing a theoretical basis for targeted clinical emergency treatment of acute decompression sickness; 3. The pathogenesis of decompression sickness was systematically studied through animal ethology, histopathology, cytology, molecular biology, microRNA chip and other technologies. A new insight is proposed that the cascade reaction of air bubbles causing pulmonary vascular endothelial damage-activation of inflammatory factors-adhesion molecule mediated-activation of coagulation and fibrinolytic system is the pathogenesis of decompression sickness caused by rapid compression and decompression, and a multi-factor comprehensive evaluation system based on reasonable weight assignment method is proposed, which provides an objective and scientific evaluation basis for research on the prevention and treatment of decompression sickness; 4. Using experimental methods such as gene chips and metabolomics, it was discovered for the first time that drugs such as inosine, clopidogrel, N-acetylcysteine, and pyrrolidine dithiocarboxylic acid can regulate metabolic products, reduce apoptosis, inhibit inflammatory reactions, and improve microcirculation, inhibit platelet aggregation and activation to prevent the occurrence of decompression sickness. New methods for preventing decompression sickness in diving operations including medication and oxygen inhalation are comprehensively proposed; 5. For the first time in China, a new comprehensive treatment plan for decompression sickness, including 0.12MPa oxygen inhalation therapy, has been proposed, which solves the problem that the diving operation site cannot be equipped with steel pressurized chambers, resulting in the inability to treat decompression sickness patients in time. It has been applied clinically and achieved Good results; 6. Using new theories and technologies in biology, diving medicine, and hygiene, we have developed the world's most stressful world. The high-pressure cell incubator (working pressure 200 meters, international 60 meters) has established a research platform for cells to change under different high pressure pressures and different exposure times, solving the problem that previous cytological research can only be exposed to high pressure for a short period of time, and the actual diving operation conditions differ greatly and cannot reflect the actual working conditions of diving operations, providing a practical and reliable research technology and platform for conducting diving medical research at the cell level. It has obtained 6 national invention patents and 3 utility models. The national military standard of the "Collective Rescue Medical Support Regulations" has been approved. Published more than 30 papers and 8 SCI papers, which he cited 42 times. The research results have been widely used in Yantai Salvage Bureau, Naval Submarine Academy, Naval Engineering University, Theater Naval Health Department, Military and Outside Hospitals, Tunnel Companies and other units. It has greatly reduced the incidence of decompression sickness during operation, significantly improved the treatment rate of decompression sickness during clinical and diving sites, and achieved significant social, economic and military benefits.